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文献和实验ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
over a piece of black cardboard, using a P-200 Gilson pick colony bringing with it the smallest possible volume of PBS. Pick 6 colonies in succession into a 96 well plate containing 20ul TRYPSIN/EDTA. Check using microscope that there are single colonies
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
ul TRYPSIN/EDTA. Check using microscope that there are single colonies in each well. Using a multichannel pipette colonies are dispersed by pipetting up and down 40x in a circular motion being careful not to make bubbles as these cause cell death, check
Fibronectin Sigma F0895-2MG Glass Bottle (250ml) with Cap Cole Parmer Instrument Co. 34594
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