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文献和实验cell strain for subculturing and biochemical analysis. These workers tested both recrystallized trypsin and NF 1:250 trypsin for cell harvesting and found that the purified trypsin was more potent and less toxic to cells. Nevertheless the NF 1:250
胰酶消化修复抗原 Trypsin Digestion of Paraffin sections prior to Immunohistochemistry 1. Preheat the following to 37oC using a water bath: (i) 200ml of TBS (ii) 200ml of distilled water. 2. Dissolve 0.2g Trypsin 250 and 0.2g Calcium Chloride
also contains a pouch of Leibovitz L-15 Media Powder. Reconstitute the entire contents of the pouch into a beaker containing 800ml of cell culture grade water. Rinse pouce 2-3 times with additional 100ml. Bring total volume to 1 liter and filter through a 0.22
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