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上海玉博生物科技有限公司
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文献和实验Methods2.1. Material and reagentsDuolbecco’s phosphate buffered saline (PBS), Medium 199 with Earle’s salts and 25mM HEPES, collagenase Type II (from Clostridum histolyticum), antibiotic/antimyotic solutuion (containing 10,000U penicillin, 10,000µg
or anhydrous salts c S. Ringer, J. Physiol. (London) 18 , 425 (1895) d M.V. Tyrode, Arch. Int. Pharmacodyn. Ther., 20 , 2025 (1910
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
-treatment conditions.Histochem . J. 18 :271. Rodriguez, J., and F. Deinhardt. 1960. Preparation of a semipermanent mounting medium for fluorescent antibody studies. Virology 12 :316. Rosenberg, W.S., X.O. Breakefield, C. DeAntonio, and O
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