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RAT/MOUSE GROWTH HORMONE ELISA KIT

, 10 µL Rat/Mouse Growth Hormone Standards in the order of ascending concentration to the appropriate wells. Add in duplicate, 10 µL QC1 and 10 µL QC2 to the appropriate wells. Add sequentially, 10 µL of the unknown samples in duplicate

Transgenic Mouse and Transgenic Rat Outline

into fertilized mouse or rat eggs, the Transgenic Core requires that you provide evidence that you have a PCR or Southern blot assay that detects your transgene when it is spiked into tail DNA at a one copy concentration. A second assay that will detect

Quantification of Gene Transcripts with Deep Sequencing Analysis of Gene Expression (DSAGE) Using 1 to 2 µg Total RNA

analysis of gene expression (DSAGE) measures global gene transcript levels from only 1 to 2 µg total RNA by massively parallel sequencing of cDNA tags. This unit describes the construction of 21?bp cDNA tag libraries appropriate for massively parallel