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文献和实验Protocol for 5' End Labeling RNA
(available in the KinaseMax™ Kit) or by the following protocol: 3) To the above reaction, add: 125 µl water 15 µl 5.0 M ammonium acetate or 3.0 M sodium acetate 150 µl phenol/chloroform Phenol extract by spinning the tube at room
Isolation of Total RNA from Difficult Tissues
techniques to help overcome problems associated with isolating total RNA from difficult tissues. The tips provided are based on RNA isolation by a guanidinium thiocyanate/acid phenol:chloroform extraction method (e.g. Ambion"s ToTALLY RNA™ Kit). Note
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
with the reagent. As a positive control, use cells set aside at the last step of the reprogramming procedure. b. Carry out a reverse transcription reaction using 1 µg of RNA (from step 1, above) and the SuperScript® VILO™ cDNA Synthesis Kit following
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