- ￥3000
- ATCC
- ATCC9372
- 2025年07月06日
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文献和实验RNA FISH on cultured cells in interphase
on the cell type. RNA is vulnerable to degradation at this step, therefore Vanadyl Ribonucleoside Complex (10mM final concentration) can be included in the extraction buffer; Incubate slides for 30 seconds in CSK buffer at room temperature; Fix
Sequential RNA and DNA fluorescence in situ hybridization
PBS with 70% ethanol and keep cells at -20°C until use (note 1); RNA FISH Wash cells with TN buffer 2 times at RT; Then incubate cells in TN buffer for 10 minutes at RT; Digest cytoplasm with 0.01% pepsin/0.01N HCl
Sequential RNA and DNA fluorescence in situ hybridization
PBS with 70% ethanol and keep cells at -20°C until use (note 1); RNA FISH Wash cells with TN buffer 2 times at RT; Then incubate cells in TN buffer for 10 minutes at RT; Digest cytoplasm with 0.01% pepsin/0.01N HCl
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