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文献和实验bodies inEscherichia coli . The current work describes a colorimetric assay for measuring RFAP synthase activity, and two modified procedures for expressing recombinant RFAP synthase genes to produce soluble, active enzyme. By lowering the incubation
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
:chloroform:isoamyl alcohol, UltraPure (25:24:1 v/v/v; Invitrogen) Phosphate-buffered saline (PBS; GIBCO 20012) Protease inhibitor cocktail (Sigma P8340) Proteinase K (10 mg/mL in TE buffer, pH 8.0; Invitrogen) PureLink HQ 96 Mini
Methods for the Measurement of a Bacterial Enzyme Activity in Cell Lysates and Extracts
C]carbamoyl phosphate was not transported into the cells, indicated that the lack of detection of enzyme activity in whole cells was a feature of the permeability of the H. pylori cell membrane, and not a deficiency of the colorimetric assay.
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