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文献和实验Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
-phosphatase labeled. (Boehringer Mannheim Cat# 1093 274)- BSA (Fraction V, Sigma Cat# A-7906)- Commercial Bleach (5.25% Sodium hypochlorite solution, Clorox)- DAPI (Sigma Cat# D-1388)- DNA from salmon testes (Sigma, Cat# D-1626) - Formaldehyde
Protocol for intracytoplasmic staining of cytokines for FACS analysis
. 2) Stimulate cells for 2 hours in vitro with 50 ng/ml PMA (Sigma, P8139) + 500 ng/ml ionomicin (Sigma I0634) in phenol red free RPMI 1640 medium + 10% fetal calf serum at 37 deg C. 3) Add Brefeldin A (Sigma, B7651) to 10mg/ml for a further 2 hr
of an overnight culture in an eppendorf tube by spinning at14K. Resuspend in 15 ml Sucrose/Tris. Prepare Lysozyme Mixture (10mg/ml (Sigma #L6876) in Sucrose/Tris) Prepare boiling water bath • Add 100 ml Triton Lysing Mix and briefly vortex on half speed. Add 10
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