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上海玉博生物科技有限公司
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文献和实验Preparation of Genomic DNA from Bacteria- using Phase Lock GelTM
Preparation of Genomic DNA from Bacteria - using Phase Lock GelTM (Modified from Experimental Techniques in Bacterial Genetics, Jones and Bartlet, 1990)
Single tube confirmation PCR protocol
(final elongation) 5. Agarose gel electrophoresis - Add 10 µl 6x loading buffer (30% glycerol, 50mM EDTA, 0.25% bromophenol blue) to the 50 µl PCR reaction. - Load 10 µl on a 1.5% agarose gel.
Introduction to the EMSA (Gel Shift) Technique
to minimize the electrophoretic dead time required for the free DNA to enter the gel matrix, especially when analyzing labile complexes.
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