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Amylose Affinity Chromatography of Maltose-Binding Protein: Purification by both Native and Novel Matrix-Assisted Dialysis Refolding Methods

Maltose-binding protein (MBP) is a carrier protein for high level recombinant protein and peptide production from either the cytoplasm or periplasm of Escherichia coli . The affinity matrix for purifying MBP-passenger proteins utilizes

【Jounal club】常用标签Tag的比较: 纯度，收率，载量，成本等

of eight elutable Affinity tags to purify proteins from Escherichia coli, yeast, Drosophila, and HeLa extracts. Our results show that the HIS, CBP, CYD (covalent yet dissociable NorpD peptide), Strep II, FLAG, HPC (heavy chain of protein C) peptide tags, and the GST

科研人必看：多肽、蛋白质、重组蛋白分不清？ 应用场景有哪些？定制如何选？

结构被广泛用于靶向脂质体、纳米颗粒及核酸递送体系构建中，用于提升体内循环稳定性并实现组织或细胞特异性摄取。 在纳米递送体系中，DSPE-PEG-多肽偶联物常与细胞穿膜肽 (Cell-Penetrating Peptide, CPP) 协同使用，以同时实现体内稳定性与细胞内高效递送，例如在其表面引入 TAT 肽，构建出 DSPE-PEG–TAT 共修饰纳米载体，用于实现肿瘤或组织中的高效细胞摄取与递送。 图 5. 转铁蛋白与细胞穿膜肽双功能修饰肽用于体内外递送质粒以治疗阿尔