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Amylose Affinity Chromatography of Maltose-Binding Protein: Purification by both Native and Novel Matrix-Assisted Dialysis Refolding Methods

Maltose-binding protein (MBP) is a carrier protein for high level recombinant protein and peptide production from either the cytoplasm or periplasm of Escherichia coli . The affinity matrix for purifying MBP-passenger proteins utilizes

【Jounal club】常用标签Tag的比较: 纯度，收率，载量，成本等

of eight elutable Affinity tags to purify proteins from Escherichia coli, yeast, Drosophila, and HeLa extracts. Our results show that the HIS, CBP, CYD (covalent yet dissociable NorpD peptide), Strep II, FLAG, HPC (heavy chain of protein C) peptide tags, and the GST

Use of Flow Cytometric Methods to Quantify Protein‐Protein Interactions

screening on 74 clones derived from the mouse in panel A. Notice the high specificity for the target antigen (RGS19) over MBP or RGS4‐MBP fusion protein. This method allows a large number of hybridomas (>200) to be screened in a single day for antigenic