Greiner Bio-One特约代理商|604181 Gr

Isolation of Untouched Human B Cells by Depletion of Non-B Cells

using a pipette with a narrow tip opening, (e.g. a 1000 μl pipette tip or a 5 ml serological pipette). 9)        Add 5 ml Isolation Buffer (When working with volumes 10)    Place the tube in the magnet for 2 min. 11)    Transfer

Isolation of Untouched Human CD8+ T Cells from Peripheral Blood Mononuclear Cells (PBMC)

-bound cells by vigorously pipetting >10 times using a pipette with a narrow tip opening (e.g. a 1000 μl pipette tip or a 5 ml serological pipette). 10)    Add 5 ml Isolation Buffer. (When working with volumes 11)    Place the tube

优化生物工艺研发的创新性技术--定量分析蛋白聚集体

后会形成蛋白聚集体。聚集信号由聚合体和单体混合比例不同决定的，但总的lgG蛋白浓度仍然是60 ug/ml。在50 mM磷酸钾，pH 7，3uM ProteoStat 检测试剂或5uM Thioflavin T 染料中读数。这些浓度在以前做过预实验，已经确定是最优的浓度。   在蛋白和染料一起孵育15分钟后使用BioTek Synergy Mx Microplate Reader读数。所用的板为Greiner μClear® black, clear bottom 96