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SSR GEL and Silver Staining Protocol

is loaded in a well separate from poly A. We do not use poly A for fingerprinting gels. Using a 144-well, microtiter 4X offset comb, load 3 μl of the mix: 3.4 μl 1X Perkin Elmer II PCR buffer 8.6 μl of 30 ng / μl PGEM 12 μl of SSRP buffer heat 95o C

PCR generalities（Components, PCR cycle, vials）

1.0µL 100 ng/25 µL * The PCR buffer used was made after the recommendations of the manufacturer/vendor (Perkin Elmer Cetus). The 10x PCR buffer contains: 500 mM KCl; 100 mM Tris-HCl (pH 8.3); 15 mM MgCl2 (the final

【共享】慢病毒载体

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