Qiagen特约代理商|34964 polypropylen

Column Buffers: CREB

.27. Column Buffers Make 500mls equivalent of base buffer: 10ml 1M Hepes pH 7.9 100ml 10% glycerol 5ml 10% NP40 (or 5.0ml 300mM Octyl glucoside) 200ml 0.5 EDTA ------- 115.2ml /5 = 23ml Base 1M KCl2.5M KCl dH20 for 0 KCL 46ml 0 0 154ml = 200

Spin Column chromatography

-50 (medium) by adding appropriate amount of dry beads to 100 ml TE buffer such that the beads will swell to 50 ml. Autoclave. 1. Rock bottle gently to make a uniform slurry. 2. Place a disposable 3ml chromatography column into a 15 ml

DEAE Column

A280.Once absorbance has leveled off at zero,the column is sufficiently washed. 10)Elute column with the appropriate salt solutions. - When eluting SMase activity,elute: a)three times with (1 ml of)100 mM NaCl. b)two times with (0.7 ml)of 250 mM