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QIAGEN Plasmid plus Kit——超快纯化多至10 mg转染级别纯质粒

      ♦利用真空方式超快速进行质粒纯化，碱裂解步骤后，利用QIAfilter无须离心即可澄清裂解液，然后离心柱安置于真空底座（例如：QIAvac 24 Plus 见图1）上进行DNA的洗涤和洗脱。Midi and Maxi kit 20 分钟内可纯化24 个样本Mega和Giga Kit可分别在40，50分钟内处理12个样本。 ♦QIAGEN Plasmid plus Midi/Maxi Kit 提供优化的高产量protocol 并采取高洗脱体积，确保纯化得到高产量质粒

Miniprep/Qiagen kit

  Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.

Plasmid

the amount of DNA obtained in a pilot experiment by comparison to standards on an agarose gel. Adjust LB culture volumes accordingly (maximum 10 ml). If culture volumes greater than 10 ml are required, use the appropriate QIAGEN Plasmid Midi, Maxi, Mega, or Giga Kit