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文献和实验CellTrace CFSE Cell Proliferation Kit
实验材料 Kit Contents CellTrace™ CFSE (Component A), 10 vials, each containing 50 μg of lyophilized powder DMSO (Component B), 1 vial containing 0.5 mL of high quality dimethylsulfoxide
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
cells/ml in culture medium in a suitable tissue culture plate or tissue culture flask. 2) Add Dynabeads Mouse T-Activator CD3/CD28 at a bead-to-cell ratio of 1:1. 3) Add 30 U/ml rIL-2. 4) Incubate in a humidified
Intracellular Cytokine Staining Protocol
A modification of the basic immunofluorescence staining and flow cytometric analysis protocol can be used for the simultaneous analysis of surface molecules and intracellular cytokines at single-cell level. In this protocol, cells
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