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文献和实验The Effect of Caffeine on the Heart Rates of Three-Day Old Chick Embryos
due to the presence of glucose as an energy source in the DMEM. Also consistent with our expectations, dilute caffeine solutions (0.1 mg/ml and 0.2 mg/ml) did further increase the heart rates relative to the base rate (see table 3). However, we did not expect
). The 10-d-old EBs are trans-ferred to adherent culture conditions and are fed with high-glucose Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% v/v fetal bovine serum, 2 mM L-glutamine, and 0.1 mM 2-mercaptoethanol for 20 d
with ddH2 O. 3) Add one packet of F-12 (HAMS) (Gibco #21700-075). 4) Stir this until all of the powder is dissolved. (Note: Do not heat to quicken the process.) 5) Once all of the media is dissolved, add 1.176-g NaHCO3 (Sodium Bicarbonate) and stir
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