gibco特约代理商|12100-061 DMEM, Pow

The Effect of Caffeine on the Heart Rates of Three-Day Old Chick Embryos

due to the presence of glucose as an energy source in the DMEM. Also consistent with our expectations, dilute caffeine solutions (0.1 mg/ml and 0.2 mg/ml) did further increase the heart rates relative to the base rate (see table 3). However, we did not expect

Derivation and Characterization of Alveolar Epithelial Cells From Murine Embryonic Stem Cells In Vitro

). The 10-d-old EBs are trans-ferred to adherent culture conditions and are fed with high-glucose Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% v/v fetal bovine serum, 2 mM L-glutamine, and 0.1 mM 2-mercaptoethanol for 20 d

Tissue Culture of PtK1 cells

with ddH2 O. 3) Add one packet of F-12 (HAMS) (Gibco #21700-075). 4) Stir this until all of the powder is dissolved. (Note: Do not heat to quicken the process.) 5) Once all of the media is dissolved, add 1.176-g NaHCO3 (Sodium Bicarbonate) and stir