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Immunization with Naked DNA Coexpressing Antigen and Cytokine via IRES

. (1 ,2 ). DNA vaccines allow the simultaneous expression of antigens and immune-stimulatory cytokines via an internal ribosomal entry site (IRES). Here we describe the construction of a DNA vaccine against malignant melanomas using: (i) the tumor-associated

Synthesis of a Virus Electrode for Measurement of Prostate Specific Membrane Antigen

. The methods described here utilize electrochemical impedance spectroscopy (EIS) to detect molecular recognition between M13 phage bound to a Au electrode and the following analytes: prostate specific membrane antigen (PSMA), positive and negative control

Using Biacore to Measure the Binding Kinetics of an Antibody‐Antigen Interaction

?protein interaction is described using an antibody and its antigen as an example. The affinity of the antibody for its antigen is determined by measuring the binding kinetics of the interaction. The protocols are divided into three major steps