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文献和实验Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
reaction will require 160-300ng of template. 1. Streak clone stock to single colony on LB (+antibiotic) plate. Inoculate 1ml LB (+antibiotic) culture with single colony, grow overnight at 37?C. Using 3 ul of (overnight growth) single colony
Analysis of methylation using bisulphite sequencing
Analysis of methylation using bisulphite sequencing Contributed by Dr. A. Gratchev This method allows precise analysis of methylation in a certain region by converting all nonmethylated cytosines into tymines, while methylated
Clone Genes From a Phage Library
The overall sequence of events is: • Titer and plate out phage • Lift plaques onto filters and prepare them for screening • Make a probe • Hybridize the probe to the filters • Wash the filters and expose to film • Purify putative plaques
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