li-cor特约代理商|927-70001 Intercep

Vybrant® DyeCycle Green and Orange Stains

material is supplied for approximately 200 fl ow cytometry assays based on a 1 mL test volume. Approximate fluorescence excitation/emission maxima: Vybrant® DyeCycle™ Green stain: 506/534 nm, bound to DNA; Vybrant® DyeCycle™ Orange stain: 519/563 nm

PBS和D-PBS缓冲溶液的配制方法

4 0.27 g （2）向烧杯中加入约800 ml的去离子水，充分搅拌溶解。 （3）滴加浓盐酸将pH值调节至7.4，然后加入去离子水将溶液定容至1 L。 （4）高温高压灭菌后，室温保存。 注意： 上述PBS Buffer中无二价阳离子，如需要，可在配方中补充1 mM CaCl2和0.5 mM MgCl2。 3. D-PBS是杜氏磷酸缓冲液 全称为：Dulbecco's Phosphate Buffered Saline

Blocking of unwanted non-specific staining

7.5, to which 0.01 Tween 20 has been added (TBS-T). 2- Briefly blot the slides without letting them dry and then apply egg white in PBS-BSA-NaN3 as a blocking agent (one egg white in 100 ml PBS + NaN3) (Miller RT et al, Appl Immunohistochemistry 5(1): 63-66