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上海玉博生物科技有限公司
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文献和实验SequalPrep Long PCR Kit with dNTPs
0.4 μl -- SequalPrep™ 10X Enhancer A or B 1–2 μl* 0.5X–1X SequalPrep™ Long Polymerase
-stop PCR only; see Steps 1-22)SSCP loading dye (for SSCP only; see Steps 23-37)95% (v/v) formamide10 mM NaOH0.25% (w/v) bromophenol blue0.25% (w/v) xylene cyanolTaq DNA polymerase (5 U/µl)TBE Buffer (1X and 5X)Prepare a 5x stock solution in 1 liter
of some templates, however 10% DMSO can reduce Taq polymerase activity by up to 50% (Gelfand 1989) so it should not be used routinely. DMSO is thought to reduce secondary structure and is particularly useful for GC rich templates. A number of PCR additives
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