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上海玉博生物科技有限公司
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文献和实验Hematoxylin and Eosin (HE) Staining
2 min Acid Alcohol x1 ("Differentiation" lightens the staining, especially outside the nucleus) Skip this differentiation step for progressive staining with the weaker Mayer's Hematoxylin stain. H2 O x1 Ammonia solution 10 dips (x
Hematoxylin and Eosin Staining
Hematoxylin, 2 min (x1) Running H2 O x 2 min Acid Alcohol x1 ("Differentiation" lightens the staining, especially outside the nucleus) Skip this differentiation step for progressive staining with the weaker Mayer's Hematoxylin stain. H2
Preparation and use of Zamboni’s-fixed Frozen Sections in Immunohistochemistry
Preparation and use of Zamboni’s-fixed Frozen Sections in Immunohistochemistry ZAMBONI’S FIXATIVE (PARAFORMALDEHYDE/PICRIC ACID) SUGGESTED PREPARATION METHOD 1. Mix 20g paraformaldehyde with 150ml double-filtered, saturated
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