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上海玉博生物科技有限公司
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文献和实验Reagent List: PCR Reagent Catalog Number Taq DNA Polymerase NEB #M0267S Nucleotide mix (dNTPs) NEB # N0447S Primers www
Inverse PCR & Cycle Sequencing
sequenced without extensive purifications. Prior to sequencing, simply pass PCR reactions through Qiagen PCR purification columns (P/N 28104) to remove primers and nucleotides and elute in 50-80 ul of 10 mM Tris. This step is essential when dye terminator
Amplification Place 50-100 ng of template DNA in a 0.2-ml PCR tube. Add forward and reverse primers to a final concentration of 0.4 µM each. Add 2.5 µl of 10X PCR amplification buffer (supplied with Taq DNA polymerase). Add dNTPs to a final concentration
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