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文献和实验【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
The availability of whole genomic sequences provides a great framework for biologists to address a broad range of scientific questions. However, functions of most mammalian genes remain obscure. The forward genetics strategy of insertional mutagenesis uses DNA
Noninvasive Human Nuclear Transfer with Embryonic Stem Cells
as described in Culturing Human Embryonic Stem Cells in Feeder-Free Conditions (McElroy and Reijo Pera 2008 ) Human oocytes In vitro matured or failed-fertilized oocytes can be used for this purpose as previously described (Lavoir et al. 2005
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
until the solution no longer turns yellow. This usually takes about 5 changes. O/N rinse is fine. View under bright field optics for optimal detection. Notes Although we have seen reduced activity of ß-gal after treatment with organic
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