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上海玉博生物科技有限公司
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文献和实验BAC DNA Purification For Injection
the syringe-reservoir with parafilm. Add 10 ml injection buffer. Start collecting 0.5 ml fractions with a 24 well plate (about 12 fractions for the dye to reach the bottom of the column). Circular BAC elutes early, about in fractions 4-7, followed by linear
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
of aggregation plate Materials: Dissecting microscope; Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3); 1 ml syringe with 26 G 1/2 needle of KSOM medium; light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);
Chick Chorioallantoic Membrane (CAM) Assay
", OD 3/32", wall 1/32") to a 3cc syringe with a 20-gauge needle. The syringe was attached to an infusion pump (Harvard Apparatus, South Natick, MA). Injection of 400 μl FITC dextran, MW 2,000,000 (Sigma, St. Louis, MO), into the umbilical vein
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