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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
dipense in 40ul aliquots in pre-chilled, sterile eppis, freeze in lN2 and store -70C AGROBACTERIUM ELECTROPORATION Safety precautions 1. Never change any other settings than stated while unit is charging (this may damage
protocol. a) Preclear cell lysates with protein G-sepharose. b) Incubate 200μg of pre-cleared lysates overnight with protein G coupled to the corresponding antibody (rotation overnight, 4°C, total IP volume 1.5 ml) c) Wash complexes 5 times
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
between chromatin elements without specifically identifying the protein components that mediate such interactions. Here we describe a detailed protocol for C ombined 3C -C hIP-C loning (6C) technology, which combines multiple techniques to identify the proteins
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