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文献和实验Extraction of Cellular and Tissue RNA
aqueous phase does not penetrate the barrier, whereas the organic phase and any residual protein interphase migrate through the barrier to become trapped underneath. Heavy grade phase-lock gel tubes (5 Prime→3 Prime) are suitable for procedures using
Fast and reliable mini-prep RNA extraction from Neurospora crassa
containing high levels of nucleases. Extraction from starved cultures or plate mycelia with the LiCl method (Chambers and Russo, Fungal Genet. Newsl. 1987. 33:22-24) gave completely degraded RNA as judged by gel electrophoresis. In trying to improve
Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period
mixture to cool to room temperature before finally correcting pH. Make up to 1 liter with distilled water. Autoclave at 15 p.s.i. for 15 min. Preparation of Red blood cell lysis buffer: 0.01 M Tris-HCl pH 7.6, 320 mM sucrose, 5 mM MgC12, 1% Triton X
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