HAMPTON research 代理商|HR2-110 C

Use of Chromophoric Ligands to Visually Screen Co‐Crystals of Putative Protein‐Nucleic Acid Complexes

slides (Hampton Research) 24‐well plastic tray (Hampton Research) Light microscope

Crystallization Trials

Materials Milipore filter type HA 0.45 micron Culture plates (Linbro model 76-033-05) Protein in DDW or HEPES buffer (10 mg/ml) Vacuum grease Syringe with 18 G needle Procedure 1.Millipore filter all solutions to be used. 2.Dialyze

Protocol for Real-Time RT-PCR

RNAaseOUT 1 ml 4. Add the reaction mixture to the RNA/primer mixture, mix briefly, and then place at room temperature for 2 min. 5. Add 1 ml (50 units) of SuperScript II RT to each tube, mix and incubate at 25°C for 10 min.