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上海玉博生物科技有限公司
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文献和实验因为自己实验后期需要使用这方面的技术,所以最近在查阅这方面的资料,想拿出来和大家一起分享.后期会把更详细的东西贴出来.英文水平一般,所以翻译的有点差,请海涵. Rac1 Activation Assay Kit被设计来鉴定贴壁细胞或非贴壁细胞的细胞裂解液中Rac活性数量(i.e. Rac-GTP, rather than the inactive Rac-GDP). 使用的glutathione (GST) pull-down方法,采用GST-agarose beads与有的包含74
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
than in controls. This indicates that the pull-down with a specific antibody enriched specific protein-occupied genomic regions. 50. Pick several white colonies (i.e., containing the insert) from the bacterial plates. 51. Use
for the siRNA targeted gene compared to the respective control(s).Part III: Detection of pRNAs by 5'biotin EF52 pull-down assay 1.A minimum of ~4.0×10 6 cells are required/sample. Twenty-four hours following the plating (~70% confluent) the cultures
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