Cytoskeleton特约代理商|BK030 RhoA /

Assessment of the Role for Rho Family GTPases in NADPH Oxidase Activation

3), but not Cdc42 or RhoA, are capable of serving as an activator of Nox1–3. Here, we describe methods for the assay of Rac binding to p67 phox and Noxa1 and for the reconstitution of Rac-dependent Nox activity in cell-free and whole-cell systems.

Biosensors for Characterizing the Dynamics of Rho Family GTPases in Living Cells

FLAIR, biosensor of Rac1 activation. This design is similar to that of the RhoA biosensor, but here the PAK fragment used to bind activated Rac1 is not part of the same chain as the Rac1. The use of a dual‐chain, intermolecular FRET design enhances

Organotypic Collagen I Assay: A Malleable Platform to Assess Cell Behaviour in a 3-Dimensional Context

.    6)      Centrifuge the extract (7,500 x g) for 30 mins and discard the pellet.    7)      Add an equal volume of 10% (w/v) NaCl to the supernatant, and stir for 30-60 min.    8)      Centrifuge (10,000 x g) for 30 mins, discard