Applichem特约代理商|A3672.0250 Dime

Cell Culture Media and Solutions

the tubes at -80 degrees C for up to one year. 700 ml RPMI-1640 with 2mM L-Glutamine 200 ml fetal bovine serum (FBS) 100 ml dimethyl sulfoxide (DMSO, Sigma) -------- 1000 ml total volume Note: Filter sterilize media and FBS

Cell Culture Protocols

vessel and the locations recorded. Key Points The most commonly used cryoprotectant is dimethyl sulphoxide (DMSO Prod. No. D2650), however, this is not appropriate for all cell lines e.g. HL60 (Prod. No. 98070106-1v1) where DMSO is used to induce

General Stem Cell Protocols

, slides, etc. It is necessary to cover the entire culture surface. 4. Freezing Medium The best recovery rate was observed in freezing medium consisting of 90% FBS-10% dimethyl sulfoxide (DMSO). However, Oct-4 expression in the thawed cells