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文献和实验In Vivo Dual Luciferase Reporter Assay with Chick Neural Tube In Ovo Electroporation System
luciferase reporter assay system (DLR, Promega, WI, USA) is designed to control for this technical issue by using a co-transfection approach with two separate reporter proteins that emit at distinct wavelengths: one from firefly (Photinus pyralis
Promoter Deletion Analysis Using a Dual-Luciferase Reporter System
different substrates to generate quantifiable luminescence. Therefore, the enzymatic activities of firefly and Renilla luciferases can be sequentially measured in a single sample by controlling reaction conditions. Here, we describe a dual-luciferase
superskyfly 我自己都觉得我自己很牛,千百人都做出来的试验,我就是做不出来。质粒用的是Promega p4.32-NF-kB-Luc, 转染用的是Invitrogen的Lipofactamin, 激活用的是Sigma生产的PMA和PHA-P,Assay用的是Promega dual,结果加了PMA和PHA的和不加的读数几乎没有区别。加了compound的和不加的也没有区别。 用过PC3, Jurkat, A549,HCT116都是一样。
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