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文献和实验Measurement of Chloride Movement in Neuronal Preparations
. Cala, P.M. 1990. Principles of cell volume regulation: Ion flux pathways and the roles of anions. In Chloride Channels and Carriers in Nerve, Muscle, and Glial Cells (F.J. Alvarez‐Leefmans and J.M. Russell eds
Competent cells, calcium chloride method, E. coli, description
preparation, most of the nucleic acid at this point is RNA). Extract the solution once with 5 ml chloroform:isoamyl solution, centrifuge and save the aqueous layer. Add 0.1 volumes of 3 M Sodium acetate, mix, overlay with 2 volumes of 95% ethanol
Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction
pipetting. 16. Add 100 μl of 3 M Sodium Acetate. Divide the sample evenly into two microcentrifuge tubes. 17. Add an equal volume of Phenol to each tube, mix well, centrifuge to separate the phases (1,000 X g), and save the aqueous phase (upper
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