gibco特约代理商|11811-031 Geneticin

Recipes for Tissue Culture Selective Drug Stocks

  Recipes for Tissue Culture Selective Drug Stocks 100X G418 (Geneticin): Obtained as crystalline powder from GIBCO (cat.# 860-1811) and stored @ room temp. in T932. The amount of active ingredient per total weight is printed

ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES

, GIBCO-BRL 11811-031) need 150ug/ml active (however, each batch should be tested. Make up X100 in PBS and filter sterilize. NB. Each batch has number of ugs/mg active written on vial, you need to allow for this when making your calculation.       FIAU

ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES

of digested DNA is enough for Southern analysis. This leaves enough for a repeat Southern if necessary. G418 (Geneticin, GIBCO-BRL 11811-031) need 150ug/ml active (however, each batch should be tested. Make up X100 in PBS and filter sterilize. NB