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文献和实验Chromatin Interaction Analysis Using Paired‐End Tag Sequencing
Analysis using Paired?End Tag sequencing (ChIA?PET) is a technique developed for large?scale, de novo analysis of higher?order chromatin structures. Cells are treated with formaldehyde to cross?link chromatin interactions, DNA segments bound by protein
Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
Reagents Buffered phenol red solution DNA plasmid of interest (e.g., GFP expression plasmid) Store DNA plasmid stocks in endotoxin-free H2 O. DNA purification kit (endotoxin-free) (e.g., Qiagen EndoFree Plasmid Maxi Kit, 12362
RNA‐Seq: A Method for Comprehensive Transcriptome Analysis
I enzyme (New England Biolabs) End‐It DNA End‐Repair Kit (Epicentre Biotechnologies) including: 10× End‐Repair
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