merck millipore特约代理商|70541-4CN

Small Scale GST-Tag Purification Under Nature Conditions

the Glutathione agarose: binding of a cell sonicate containing only GST b) If only partially bound, use more resin, or bind for longer time (The longer the duration of purification, the greater the risk of protein degradation). c) Add 1-10mM DTT prior to cell

STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS

at 37 C, sonicate 30" high, freeze lN2, thaw. 4) spin RT 10'' 15k ss34, respin in fresh tube 10'' 15k ss34. 5) decant again to new tube, add 5ml 50% Ni-NTA resin, mix gently RT 30''. 6) spin, decant and wash resin 3x in 50ml UPB6.3 w