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上海玉博生物科技有限公司
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文献和实验Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞
).remove and save polylysine.wash plates 2x with 10ml sterile water.Seed cells: 1/2 X split from confluent plate (approximately 2-5 x 106 cells per 100mm plate) in PC12 culture media.Incubate overnight in 10% CO2 incubator.Cells should be 50-70% confluent
Cell Culture Materials 1. Falcon Primaria culture dishes. 2. Culture medium: DME (or F12K) with glutamine, supplemented with 7% heat-deactivated horse serum (56oC for 30 min), 7% fetal calf serum, 50 units penicillin, 50 ug/ml
the RNA to the cellulose and mix on rotator for 1 hour at room temperature.6. Prepare a BIO-RAD Disposable Chromatography Column (BIO-RAD cat. #732-6008) by washing it out once with 750 l 1X NETS.7. Gently pour the RNA/cellulose mixture into the column
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