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文献和实验Protocol Protein Gel Staining Using Coomassie Blue Protein Stain
Materials Polyacrylamide pre-cast gel SimplyBlue™ Coomassie protein stain, 1x pre-mixed solution (Coomassie blue dye binds to proteins (arginine, the aromatic amino acids, and histidine) allowing protein bands to be visualized
1. Sections to distilled water. 2. Stain in Fouchets reagent 5 minutes. 3. Rinse in distilled water. 4. Counterstain with van gieson 5 minutes. 5. Rinse quickly in absolute alcohol. 6. Dehydrate
for at least 1 year. DAPI dihydrochloride, FluoroPure™ grade (≥98% pure) 10 mg Room temperature Protect from light
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