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文献和实验Transduction Protocol for Adherent Cell Types
efficiency may vary based on cell type. Optimization of conditions may be needed to maximize expression. 下载本文PDF格式： http://www.bbioo.com/Soft/2007/1120.htm **Polybrene or Protamine Sulfate may be used
LPD Nanoparticles-Novel Nonviral Vector for Efficient Gene Delivery
hosphatidyle thanolamine (DOPE) at a molar ratio of 6:4. The poly cation component is added for the condensation of plasmid DNA. Polylysine first was used (1 ), and later it was changed to protamine sulfate for improved activity (2 ). The optimal composition
Differentiation of Human Embryonic Stem Cells to Cardiomyocytes on Microcarrier Cultures
Protamine sulfate solution: Dissolve 32 mg protamine sulfate (Sigma, cat. no. P3369) in 4 ml coupling solution (concentration: 8 mg/ml) Blocking solution: 0.1 M Tris⋅Cl
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