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文献和实验Vybrant® DyeCycle Green and Orange Stains
Do not use glass containers with this stain Do not wash or fix cells after staining cells with Vybrant® DyeCycle™ stains Validate flow cytometry instrument performance on the day of use Use linear amplification for DNA content
Membrane Transfer and Crosslinking for RNA
UV crosslinkers. If a calibrated instrument is not available, it is possible to use standard laboratory equipment such as transilluminators and handheld ultraviolet lamps to fix RNA targets to a membrane. Care must be taken not to under or overexpose
Vybrant® DyeCycle Violet Stain
leads to significant differences in fluorescence signal. If Vybrant® DyeCycle™ Violet stain is used in combination with other stains for multicolor applications, apply the other stain(s) to the sample first, following all manufacturers
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