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文献和实验Detection Of Cell Viability And/Or Apoptosis By Flow Cytometry (FACS)
increases, DNA (and depending on the reagent, RNA too) of dead cells, necrosing cells and cells in late apoptosis will bind reagents such as propidium iodide (PI ; MW 668) and 7-actinomycin D (7-AAD ; MW 1270) to fluoresce more. Because of loss of cell
LIVE/DEAD® Violet Viability/Vitality Kit
viability and cell vitality depends on these physical and biochemical properties of the cells. As cells die, variations in fluorescence will be observed. 实验试剂 Table 1. Contents and storage information
. Resuspend cells in appropriate medium or buffer. Quantitate cell yield and viability. Seed cells for culture, if required. Perfusion procedures require special equipment and techniques
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