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上海玉博生物科技有限公司
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
of 100 units/µL). Incubate with gentle shaking overnight at 37°C. 17. Add 40 µL of 20% SDS (to a final concentration of 1.6%). Incubate with shaking for 30 min at 65°C. 18. Transfer the sample to a 15-mL tube containing
Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period
100. Add 10 ml of 1 M Tris, 109.54 gr of sucrose, 1.01 gr MgC12, adjust pH to 8.0 and finally add 10 ml of Triton X-100 to 800 ml of distilled water, and make up to 1 liter with distilled water. Autoclave at 15 p.s.i. for 10 min. Sugars at high
5030) CO2 cell culture incubator 50 ml Nunc/Falcon tubes 15 ml Nunc/Falcon tubes KCl (0.075 M, 0.055% ?) Fixative (methanol/acetic acid 3 : 1) glass microscopy slides Amounts per 5 ml blood: 40 ml RPMI 1640 Medium 10 ml FCS (20%)
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