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文献和实验of tumor cells flowing in a parallel plate chamber to determine their adhesion stabilization lag time. Cell. Motil. Cytoskeleton. 26, 88-98, doi:10.1002/cm.970260109 (1993). 21. Wiese, G., Barthel, S.R., & Dimitroff, C.J. Analysis of Physiologic E
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
/well of GIG into alternating rows (A, C, E, G) and GAM into the remaining rows (B, D, F, H). In this step, and following incubations, place a thin strip of parafilm over the groove where the lid and bottom of plate meet to prevent evaporation of solutions
ELISA (Enzyme-Linked ImmunoadSorbent Assa
is from colourless to blue (yellow when acid is added). 2) Coat plate with antigen stock solution (1ug/ml in PBS, 50ul/well) for at least 24hrs or over the weekend at 4°C or O/N at 37°C. 3) Remove antigen and wash 3x with 1x PBS. 4) Block with 1% BSA (100
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