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上海玉博生物科技有限公司
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文献和实验In Situ Hybridization Using Digoxigenin Labeled Probes
and Washes: 1) Denature the hybridization probe by boiling for 3 minutes and chill on ice 2) Apply 5 µl of hybridization solution on the slides and distribute the solution over the chromosomes with a 18 x 18 mm coverslip. Try to avoid trapping air
Gradient‐type HPLC system, preferably equipped with a PDA detector Reversed‐phase HPLC column [Waters µBondasphere C18 5 µm 300A (3.9 ×150–mm) or XBridge C18 5 µm
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
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