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- 供应商:
上海玉博生物科技有限公司
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文献和实验(主要针对Miltenyi产品) 一、标记策略 用MACS进行磁分离,最重要的参数就是高质量的标记。对阳性细胞的标记应尽可能强,而背景要尽可能低,才能获得对磁标记的阳性细胞和未标记的阴性细胞的最好分辨。有多种不同的标记策略可供参考: 1、直接标记
Mouse B cell isolation with magnetic beads
9.Constant volume to 20 ml/ each spleen with MACS, and transfer to another tube through a new strainer, centrifuging for 5 min, 4℃; Resuspend cells with MACS to 1 ml/ each spleen. 10.Follow Miltenyi Biotec protocol of column
Isolation and Propagation of Mouse Liver-Derived Dendritic Cells
Dendritic cells (DC) are highly specialized antigen-presenting cells (APC) derived from precursors within the bone marrow (BM). They are distributed ubiquitously throughout the body, and are few in number (1 ). They are classified
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