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- miltenyi biotec
- 130-093-130
- 2025年07月15日
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上海玉博生物科技有限公司
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文献和实验Mouse B cell isolation with magnetic beads
9.Constant volume to 20 ml/ each spleen with MACS, and transfer to another tube through a new strainer, centrifuging for 5 min, 4℃; Resuspend cells with MACS to 1 ml/ each spleen. 10.Follow Miltenyi Biotec protocol of column
Th17 Polarization of Mouse CD4 Cells
of anti-mouse CD28, clone 37.51(5μg/mL),IL-6 (50ng/mL),TGF-β1 (1ng/mL), anti-mouse IL-4 (10μg/mL), anti-mouse IFN-γ (10μg/mL), and 300 nM of FICZ. Alternatively, mouse IL-23 (5ng/ ml) can be used in place of FICZ. 3) On day
Dual- and Triple-Color Fluorospot
antibodies (mAbs) to cytokines: Mouse mAb to human IFN-γ; mAb 1-D1K for coating and FITC-labeled mAb 7-B6-1 for detection. Mouse mAb to human IL-2; mAbs IL2-I/249 for coating and biotinylated mAb IL2-II for detection. If a third cytokine is included
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