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文献和实验Chromatin IP (CHIP assay) This protocol has some minor modification to the protocol described in Strahl-Bolsinger S. et al. [1997, Gen & Dev 11, p83-93] and was obtained from Flick K. (The Scripps Research Institute).
were washed with PBS and resuspended in ChIP lysis buffer (1% SDS, 10mM EDTA, 50 mM Tris-HCl pH8.0) add protease inhibitors (inhibitors: 1mM PMSF, 1microgram/ml aprotinin and 1microgram/ml pepstatin A).. After incubated 10 minutes on ice, cells were sonicated
Chromatin Immunoprecipitation (ChIP)
The first chromatin immunoprecipitation (ChIP) assay was developed by Gilmour and Lis (1984, 1985, 1986) as a technique for monitoring the association of RNA polymerase II with transcribed and poised genes in Escherichia coli and Drosophila . The use
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