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文献和实验4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody
using an anti-4-HNE monoclonal antibody (MAb) (6 –8 ) and labeled goat anti-mouse IgG antibody (9 ).
The Peroxidase-Antiperoxidase (PAP) Method
of the peroxidase is not affected by the attached immunoglobulins. The antiperoxidase antibodies are from the same species as that which has produced a primary antibody raised against a tissue antigen. These two antibodies, one directed against the tissue antigen
ELISA Procedure for Measuring Serum Antibody Titer
. Reagents for Horseradish Peroxidase Detection1. Goat anti-species IgG whole molecule/horseradish peroxidase conjugate that is appropriate for the source of your antiserum. For example, if the antibodies were raised in a rabbit, use goat anti-rabbit IgG
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