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文献和实验for 5 min. Transfer 600 μL the supernatant into a new 2 ml tube and add 200 μL Buffer SP2. Mix the sample throughly by vortexing. 5. Incubate on ice for 5 minutes. Centrifuge at 13,000 x g in a microcentrifuge for 5 minutes. 6. Carefully
-His fusions)or 900 ml (MBP fusions)LB medium (Sambrook et al.,1989)containing kanamycin at 50 µg/ml (the plasmid vector confers resistance to this drug).The cultures were shaken vigorously at 37ºC until the optical density at 600 nm reached 0.5; IPTG
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
is precipitated by adding Protein Precipitation Reagent (PPR). After removal of the protein, the supernatant is mixed with 1 volume of isopropanol to precipitate the RNA. After the centrifugation to pellet the RNA, the supernatant is transfer to a new tube
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