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上海玉博生物科技有限公司
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文献和实验Preparation of Embryonic Extract
, on ice. 10. 12-day chicken embryos, 12. 11. Polytron with PTA 10TS generator or a Dounce homogenizer with a Teflon pestle of coarse fitting. 12. 50 ml conical disposable centrifuge tubes. 13. 50.2 Ti rotor and tubes, chilled. 14
Preparation of nuclear extract and cytoplasmic extract
PMSFBuffer D (Dialysis Buffer): For 2 L20 mM HEPES,pH 7.9 1 M HEPES 7.9 40 ml20 % Glycerol Glycerol 400 ml100 mM KCl3 M KCl66.67 ml0.2 mM EDTA 0.5 M EDTA 800 µl0.5 mM DTT 1M DTT 1 ml0.2 mM PMSF 0.2 M PMSF 2 mlSplicing Extract Preparation1.Resuspend cell
Preparation of Embryonic Extract
. 9. 25-50 ml Hank's balanced-salt solution, on ice. 10. 12-day chicken embryos, 12. 11. Polytron with PTA 10TS generator or a Dounce homogenizer with a Teflon pestle of coarse fitting. 12. 50 ml conical disposable centrifuge tubes. 13
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