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文献和实验Preparation of Embryonic Extract
of fine forceps. 4. Cut off embryo heads and discard, use 1st fine scissors. 5. Slit embryos ventrally, pierce heart 3-4x to drain blood, use 1st pair of fine scissors. Cut legs off and discard. 6. Transfer embryos to the second sterile 100
Preparation of Embryonic Extract
6. Transfer embryos to the second sterile 100 mm pertri dish on ice, use second pair of forceps. Clean up the first dish with Kimwipe. 7. Chop each embryo 4-5x with the second pair of fine scissors. 8. Divide saline G (250 ml) into 3x 250 ml beakers
Single-Molecule Tracking of mRNA in Living Cells
of chicken embryo fibroblasts. Furthermore, this chapter presents an analysis approach for single-molecule tracking data to extract quantitative information about the microenvironments of the mRNA molecules.
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